By M. Ian Phillips (Editor)
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Extra resources for Antisense Therapeutics 2nd edition (Methods in Molecular Medicine)
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Simultaneous knockdown of more than two genes is possible, as illustrated by the double suppression of the nuclear mitotic apparatus protein (NUMA1) and lamin in HeLa cells (61). However, different siRNA species can possibly undergo reversible competition in a sequence-independent manner (58,62), suggesting that the RNAi machinery might be titratable or limited in mammalian cells. 3. siRNA as an Antiviral Agent The ability of RNAi to protect plants and insects from viral infection can be applied to mammals, although mammalian cells themselves do not possess inherent RNAi mechanisms.
Int. J. Oncol. 20, 745–752. 14. , Fenske, D. , and Cullis, P. R. (2001) Developments in liposomal drug delivery systems. Expert Opin. Biol. Ther. 1, 923–947. 15. , and Couvreur, P. (2002) Nanoparticles in cancer therapy and diagnosis. Adv. Drug Deliv. Rev. 54, 631–651. 16. 16 Manoharan, M. (2002) Oligonucleotide conjugates as potential antisense drugs with improved uptake, biodistribution, targeted delivery, and mechanism of action. Antisense Nucleic Acid Drug Dev. 2, 103–128. 17. , Warnier, M.
Antisense Therapeutics 2nd edition (Methods in Molecular Medicine) by M. Ian Phillips (Editor)