Download PDF by M. Ian Phillips (Editor): Antisense Therapeutics 2nd edition (Methods in Molecular

By M. Ian Phillips (Editor)

ISBN-10: 1588292053

ISBN-13: 9781588292056

ISBN-10: 1592598544

ISBN-13: 9781592598540

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Extra resources for Antisense Therapeutics 2nd edition (Methods in Molecular Medicine)

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Sci. USA 99, 6047–6052. 90. 90 Paddison, P. , Caudy, A. , et al. (2002) Short hairpin RNAs (shRNAs) induce sequence-specific silencing in mammalian cells. Genes Dev. 16, 948–958. 91. 91 Brummelkamp, T. , and Agami, R. (2002) A system for stable expression of short interfering RNAs in mammalian cells. Science 296, 550–553. 92. 92 Paul, C. , Good, P. , and Engelke, D. R. (2002) Effective expression of small interfering RNA in human cells. Nat. Biotechnol. 20, 505–508. 93. 93 Miyagishi, M. and Taira, K.

Simultaneous knockdown of more than two genes is possible, as illustrated by the double suppression of the nuclear mitotic apparatus protein (NUMA1) and lamin in HeLa cells (61). However, different siRNA species can possibly undergo reversible competition in a sequence-independent manner (58,62), suggesting that the RNAi machinery might be titratable or limited in mammalian cells. 3. siRNA as an Antiviral Agent The ability of RNAi to protect plants and insects from viral infection can be applied to mammals, although mammalian cells themselves do not possess inherent RNAi mechanisms.

Int. J. Oncol. 20, 745–752. 14. , Fenske, D. , and Cullis, P. R. (2001) Developments in liposomal drug delivery systems. Expert Opin. Biol. Ther. 1, 923–947. 15. , and Couvreur, P. (2002) Nanoparticles in cancer therapy and diagnosis. Adv. Drug Deliv. Rev. 54, 631–651. 16. 16 Manoharan, M. (2002) Oligonucleotide conjugates as potential antisense drugs with improved uptake, biodistribution, targeted delivery, and mechanism of action. Antisense Nucleic Acid Drug Dev. 2, 103–128. 17. , Warnier, M.

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Antisense Therapeutics 2nd edition (Methods in Molecular Medicine) by M. Ian Phillips (Editor)


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